Identification of Mesenchymal Stem Cell Gene Markers for Quality Control
Mesenchymal Stem Cell, Biomarker, Serum Free Culture Medium
Graduate School of Advanced Sciences of Matter Department of Molecular Biotechnology
email@example.com Basic Biology、 Chromosome Dynamics
and Synovial MSC (SY-MSC) culture systems to transplant for
clinical osteo/chondrocytes regeneration by using serum free
culture medium, STK. The STK medium is chemically defined
and the MSCs were more proliferative under the STK, so that
we aim to confirm the system is safe and highly efficient for
need to set up biomarkers of cultured MSCs for quality control.
We have already examined gene expression profiles of BMMSCs
cultured in serum containing medium and screened the
candidate gene markers by examining their gene expression
levels of more than 40 human BM-MSC lines. In this on going
project, we are searching for candidate gene markers of SYMSCs
in the following 2 steps.
Step 1) Set up gene markers of SY-MSC cultured in serum
containing medium. A) By examining mRNA expression of gene markers set up in the BM-MSCs cultured in serum
containing medium. B) By analyzing gene expression profiles using DNA microarray technique.
Step 2) Set up gene markers of SY-MSC cultured in STK medium by the methods shown above.
test samples of BM-MSCs, SY-MSCs, and Fibroblasts to test the candidate marker gene expression. Availability of the
serum medium cultured BM-MSC markers in SY-MSCs is still tested. We are also collecting additional test SY-MSC
samples from donated synovial tissues for future examination.
technique conventionally. However, this methods needs many numbers of MSCs to judge the cell quality. Our strategy
has advantages in the following points,
1) We just need less number of the cells for quality control. 2) we can set up the gene markers that ensure the SYMSC
quality based on characteristics of the MSCs, such as undifferentiated status/stemness, non contamination of
other cell source, non malignancy, etc.